Hi Philip; *Very* interesting about being able to study fossilized coprolites! I've never heard about that. It would be interesting to see content data from any study.....after a little googling I found these: From http://www.mcmaster.ca/research/sciencecity/globe-article_poinar.htm ."It's one of the biggest crap deposits known," says Vaughn Bryant, an anthropologist at Texas A&M University who led the excavation of the Hinds Cave deposit in the mid-1970s and provided Dr. Poinar with the samples. The cave, an enormous, very dry, cliff-face rock shelter, housed generations of hunter-gatherers for 9,000 years. The site has yielded more than 2,000 cow-patty-shaped human coprolites. The shape of these coprolites is due to the "astronomical" amounts of fibre in them, Dr. Bryant says. He estimates that the Hinds Cave inhabitants ate 15 times the daily fibre intake of present-day North Americans, mostly in the form of roasted desert plants, including agave and yucca. Using mitochondrial DNA analysis, Dr. Poinar showed that three coprolites belonged to separate individuals. And he confirmed Dr. Bryant's microscopic analysis of the contents: These paleo-peoples were eating well. Through genetic reconstruction, he showed that in the 24 to 48 hours before relieving himself at the back of the shelter, one Hinds Cave resident had eaten a veritable Thanksgiving feast. The coprolite included evidence of pronghorn antelope, cottontail rabbit, packrat, squirrel and eight types of wild plants. * * * http://www.scirpus.ca/dung/human.htm Callen, E. O., and T. W. M. Cameron 1960 A Prehistoric Diet Revealed in Coprolites. The New Scientist 18(1, 7 July):35-40. AEU SCI Q 1 N53 A classic paper; the beginning of scientific analysis of coprolites. Reports on the examination of coprolites from Huaca Prieta, Peru. Describes techniques used to rehydrate and examine coprolites. Shows that diet contained sea- food (shellfish, crabs, sea urchin) which was a major dietary component; not surprising for a site in a coastal location. Found seed of Capsicum (pepper), probably chili, also remains of beans (epidermal cells), identified as Phaseolus (green beans or lima beans) and Canavalia. Suggest that whole bean pods were eaten fresh. Some plant tissue identified as from cucurbit (squash); rinds and seeds were found in archaeological context. Long discussion as to what part of the squash was eaten; some authorities believe that only the seeds were roasted and eaten not the flesh. Perhaps some evidence of roots and tubers, as fibres were also found in coprolites. Reports on the abdominal contents of skeletonised body found in the deposits. Evidence for a varied diet including plant and sea-food; shows that more than one food type was consumed at a meal. Examined samples for parasite remains, found possible Diphyllobothrium (tapeworm) eggs in one sample. (31/03/2002). Dickson, J. H., K. Oeggl, T. G. Holden, L. L. Handley, T. C. O'Connell, and T. Preston 2000 The Omnivorous Tyrolean Iceman: Colon Contents (Meat, Cereals, Pollen, Moss and Whipworm) and Stable Isotope Analyses. Philosophical Transactions of the Royal Society London, Series B 355(29th December):1843-1849. Written to refute conclusions of other studies (which were based on isotopic composition of hair samples) that the diet of the Iceman was primarily vegetarian. This paper describes the remains recovered from the colon contents, which include meat remains, bran, cereal, plant remains, Trichuris (whipworm) eggs, and also pollen. Pollen includes that from hophornbeam (Ostrya carpinifolia) and hazel (Corylus avellana). It is possible that these were ingested through drinking water, and implies a spring/early summer time of death rather than autumn as implied by previous work. Farrell, N. 1988 Chapter 10: Analysis of Human Coprolites from CA-RIV-1179 and CA-RIV-2827. In Archaeological Investigations at CA-RIV-1179, CA-RIV-2823, and CA-RIV-2827, La Quinta, Riverside County, California, edited by M. Q. Sutton and P. J. Wilke, pp. 129-142. Archives of California Prehistory, Number 20. Coyote Press, Salinas, California, USA. Human coprolites, 128 specimens, some fragmentary, were recovered from CA-RIV-1179. Chapter reports on analysis of 30 of them. Also included 3 human coprolites from CA- RIV-2827. Most abundant plant material was cattail (Typha spp.) in 21 samples from CA-RIV-1179, especially anthers. Cattail pollen may have been being consumed, as is reported in the ethnographic literature. Most other seed types were present in only trace amounts in a few specimens. Considerable amounts of fish remains were found. Fish bone (not all identifiable) was found in all 30 specimens from CA-RIV-1179. Most common taxon was Colorado River bonytail chub (Gila elegans) in 12 specimens from CA- RIV-1179. Some specimens also yielded mammal, tortoise, and reptile bone fragments. Very few food items apparently consumed. May indicate that sites were seasonal camps. (13/07/2006) . Fry, G. F. 1977 Analysis of Prehistoric Coprolites from Utah. University of Utah Anthropological Papers Number 97. University of Utah Press, Salt Lake City, Utah, USA xi + 45 pp.. AEU HSS E 51 USS No 097 Two of the sites discussed are in NW Utah, west and north of the Great Salt Lake (Danger Cave and Hogup Cave). The third locality is Glen Canyon along the Colorado River, in SE Utah. These are rock shelter sites, and 15 are included in this study. Glen Canyon sites are mostly Fremont and Anasazi (around 2000 yr BP to 700 yr BP). All coprolites examined are thought to be of human origin. Report includes description of methods used for rehydration (using trisodium phosphate solution), screening and counting (pp. 7-9) for diet studies and a procedure for extracting parasites. Some coprolites were subsampled for pollen; these are the samples reported by Kelso (1970). Results from Hogup and Danger Caves show essentially similar subsistence for millennia. Main component is chenopod seed (Allenrolfea occidentalis, pickleweed). Fremont and Shoshoni samples show slight increased utilisation of Gramineae seed. Opuntia remains are also common throughout. Usually the pads would have been eaten, with the spines singed off first. Compares results with ethnohistoric evidence from the area. Coprolites show evidence that small mammals were eaten and finely divided plant material suggests that stomach contents of large herbivores were also consumed (in Fremont samples). Grit shows that seeds were probably ground before being consumed. Poinar, H. N., M. Kuch, K. D. Sobolik, I. Barnes, A. B. Stankiewicz, and T. Kuder (and W. G. Spaulding, V. M. Bryant, A. Cooper, S. Pääbo) 2001 A Molecular Analysis of Dietary Diversity for Three Archaic Native Americans. Proceedings of the National Academy of Sciences 98(8):4317- 4322. Examined three coprolites from Hinds Cave (Texas). Amplified DNA sequences from them and matched against database of known samples for plants and animals. Samples were AMS dated; all yielded dates falling in range 2100 - 2400 yr BP. Checked to see if samples of human origin by examining mitochondrial DNA; results showed a good match with contemporary Native America mtDNA, also indicated that samples were from three different people. Wide range of plant families identified including Liliales, Asteraceae, Ulmaceae, Fagaceae, Solanaceae, Fabaceae, Fouquieriaceae, and Rhamnaceae. Examination of plant macroremains in samples showed Liliaceae, Fabaceae, and Ulmaceae. Interestingly, these also shows remains of Cactaceae, which was not found in DNA analysis. Animal sequences showed match to Ovis (sheep), pronghorn, and cottontail rabbit. Macroremains analysis showed remains of small mammals and fish; perhaps large mammals not identified because meat, not bone, consumed, so their macroremains not found in coprolites. Results showed a varied and diverse diet. Diet included hackberry, sunflower family, yucca or agave, opuntia, oak, legume family, nightshade family, ocotillo, buckthorn family, and meat from at least pronghorn, rabbit, bighorn sheep, packrat, squirrel, fish, and cottontail. Each sample contained a variety of food types. (14/04/2002). * * * My impression is that whatever was around and edible, was eaten which typically involved a blend of meat and vegetable matter. An aside: another URL that deals with fibre and cancer from plant remains http://www.nature.com/ejcn/journal/v61/n1/full/1602486a.html From my limited reading it seems that they are only able to analyze fossils not older than around 10,000 years although I am not sure about that.... This is a fascinating area to further research, Philip. Marilyn