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Anyone have any success using enzymes for gluten intolerance (see abstract below). My son has not been tested yet, although he does have positive IGG. We are looking at using enzymes to see if there is any difference.
kathy
European Food Research and Technology
http://link.springer.de/link/service/journals/00217/bibs/9209006/92090428.htm
ISSN: 1438-2377 (printed version)
ISSN: 1438-2385 (electronic version)
Table of Contents
Abstract Volume 209 Issue 6 (1999) pp 428-433
Screening methods for the proteolytic breakdown of gluten by
lactic
acid bacteria and enzyme preparations
Karina Wehrle (1), Noeleen Crowe (2), Ineke van Boeijen (2), E.
K. Arendt (2)
(1) Department of Food Science and Technology and National Food
Biotechnology Centre, University College Cork,
National University of Ireland, Cork, Ireland
(2) Department of Food Science and Technology, University College
Cork, National University of Ireland, Cork,
Ireland e-mail: [log in to unmask]
Received: 25 February 1999
Abstract Gluten is the major protein in wheat and it is largely
responsible for the rheological characteristics of bread
dough. The sourdough process is a traditional dough fermentation
process with lactic acid bacteria. The proteolytic
activity of lactic acid bacteria derived from starter cultures
used in sourdough or meat fermentation was tested on
gluten as a substrate. The activities of commercial enzyme
preparations, which were derived from bacterial or fungal
sources, were also evaluated. Proteolytic breakdown of gluten
protein in an agar medium by either bacterial culture or
enzymes was evident by a clear zone around wells after staining
with Coomassie blue. The increase in TCA-soluble
material due to gluten breakdown was measured
spectrophotometrically. A third test evaluated the release of free
amino acids due to exoproteolytic activity. The agar based test
showed positive results for the commercial enzyme
preparations and one Micrococcus strain. All sourdough strains
were positive in the enzyme test whereas some
starters from the meat fermentation process were not able to
break down gluten under test conditions. Variations in
the release of free amino acid indicated differences in the
enzyme systems of the lactic acid bacteria tested.
Key words Proteolysis · Gluten · Sourdough · Starter · Lactic
acid bacteria
Article in PDF format (512 KB)
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