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Fri, 21 Feb 1997 08:41:26 -0800 |
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In my recent post about food allergy testing, I mentioned
that I had an ELISA test. Here's a definition of ELISA
from Mosby's Medical, Nursing, and Allied Health
Dictionary. It gets a little medical-technical.
Nancy
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Enzyme-linked immunosorbent assay (ELISA)
A laboratory teechnique for detecting specific antigens or
antibodies, using enzyme-labeled immunoreactants and a
solid-phase binding support, such as a test tube. A number of
different enzymes can be used, including carbonic anhydrase,
glucose oxidase, and alkaline phosphatase. Labeling is
done by covalently binding the enzyme to the test
substance through an enzyme-protein coupling agent,
such as glutaralydehyde. Products of the reaction may be
detected by fluorimetry or photometry. ELISA is nearly
as sensitive as radioimmunoassay and more sensitive than
complement-fixation, agglutination, and other techniques.
It is commonly employed in the diagnosis of HIV infections.
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