How is this test administered? My daughter came up negaative on blood
tests for gluten and milk, but her dr (GI & nitrition sepecialist) said
that they were inaccurate and worthless. He is having her do a "Breath
Hydrrogen Test". He said it would be extremely accurate and tests for a
lot of things. Please, I'd appreciate anyones opinion. We are trying to
find a cause for her IBS, and since she had been unable to tolerate milk
since she was born, I feel that this may still be her problem.
Thank you, Michele T.
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From: Nancy Newlin[SMTP:[log in to unmask]]
Sent: Friday, February 21, 1997 11:41 AM
To: [log in to unmask]
Subject: Definition of ELISA
In my recent post about food allergy testing, I mentioned
that I had an ELISA test. Here's a definition of ELISA
from Mosby's Medical, Nursing, and Allied Health
Dictionary. It gets a little medical-technical.
Nancy
- - - - - - - - - - - - - - - -
Enzyme-linked immunosorbent assay (ELISA)
A laboratory teechnique for detecting specific antigens or
antibodies, using enzyme-labeled immunoreactants and a
solid-phase binding support, such as a test tube. A number of
different enzymes can be used, including carbonic anhydrase,
glucose oxidase, and alkaline phosphatase. Labeling is
done by covalently binding the enzyme to the test
substance through an enzyme-protein coupling agent,
such as glutaralydehyde. Products of the reaction may be
detected by fluorimetry or photometry. ELISA is nearly
as sensitive as radioimmunoassay and more sensitive than
complement-fixation, agglutination, and other techniques.
It is commonly employed in the diagnosis of HIV infections.
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