How is this test administered?  My daughter came up negaative on blood
tests for  gluten and milk, but her dr (GI & nitrition sepecialist) said
that they were inaccurate and worthless.  He is having her do a "Breath
Hydrrogen Test".  He said it would be extremely accurate and tests for a
lot of things.  Please, I'd appreciate anyones opinion.  We are trying to
find a cause for her IBS, and since she had been unable to tolerate milk
since she was born, I feel that this may still be her problem.
Thank you, Michele T.

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From:   Nancy Newlin[SMTP:[log in to unmask]]
Sent:   Friday, February 21, 1997 11:41 AM
To:     [log in to unmask]
Subject:        Definition of ELISA

In my recent post about food allergy testing, I mentioned
that I had an ELISA test.  Here's a definition of ELISA
from Mosby's Medical, Nursing, and Allied Health
Dictionary.  It gets a little medical-technical.

Nancy

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Enzyme-linked immunosorbent assay  (ELISA)

A laboratory teechnique for detecting specific antigens or
antibodies, using enzyme-labeled immunoreactants and a
solid-phase binding support, such as a test tube.  A number of
different enzymes can be used, including carbonic anhydrase,
glucose oxidase, and alkaline phosphatase.  Labeling is
done by covalently binding the enzyme to the test
substance through an enzyme-protein coupling agent,
such as glutaralydehyde.  Products of the reaction may be
detected by fluorimetry or photometry.  ELISA is nearly
as sensitive as radioimmunoassay and more sensitive than
complement-fixation, agglutination, and other techniques.
It is commonly employed in the diagnosis of HIV infections.