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Date: | Thu, 23 Mar 1995 14:50:00 EST |
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If you don't have access to a medical library, one can usually get a
reprint by sending a request by mail to the authors which are listed
below in the more complete medline output.
1
UI - 95054826
AU - Miletic ID ; Miletic VD ; Sattely-Miller EA ; Schiffman SS
TI - Identification of gliadin presence in pharmaceutical products.
LA - Eng
MH - Alcohol, Ethyl ; Blotting, Western ; *Drug Contamination ; Drugs/
*CHEMISTRY ; Electrophoresis, Polyacrylamide Gel ; Enzyme-Linked
Immunosorbent Assay ; Gliadin/*ANALYSIS ; Sodium Dodecyl Sulfate
; Solvents
RN - 0 (Drugs) ; 0 (Solvents) ; 151-21-3 (Sodium Dodecyl Sulfate) ;
64-17-5 (Alcohol, Ethyl) ; 9007-90-3 (Gliadin)
PT - JOURNAL ARTICLE
AB - Celiac disease is characterized by hypersensitivity to the
alcohol-soluble wheat proteins called gliadins. Total avoidance
of gliadin is the lifelong treatment for such patients. However,
wheat gliadins are often present as impurities in industrial
starch commonly used in the preparation of different
pharmaceutical products. Therefore, some drugs might contain
gliadin, which can be dangerous for sensitive patients if taken
in large amounts or used permanently. The purpose of this study
was to develop a sensitive, reliable assay that is specific for
the detection of gliadins in pharmaceutical products. Gliadins
were extracted here using sodium dodecyl sulfate rather than 70%
ethyl alcohol, which has been the traditional solvent. This
gliadin extract was utilized in a dot-blot assay that
incorporated an antigliadin antibody developed in rabbit and
labeled with peroxidase. 4-Chloro-1-naphthol was used as a
peroxidase-specific substrate. Isolated wheat gliadin was used as
the positive control. Dilution experiments showed that the lower
level of sensitivity for the assay was in the range of 0.0045
mg/ml of gliadin, which is a concentration level lower than that
suggested for a gluten-free diet. The assay developed here
revealed that 71.2% of 59 prescription and nonprescription drugs
tested contained gliadin in the amount detected by our dot-blot
assay. The prescription drugs tested were among the top 50 most
frequently dispensed in U.S. community pharmacies. The
nonprescription drugs were among those that constitute the
largest sales in the United States. The results showed that the
simple dot-blot assay developed here can be used for
pharmaceutical testing performed either by hospital laboratories
or by patients themselves.
AD - Department of Psychiatry, Duke University, Durham, North Carolina
27706.
SO - J Pediatr Gastroenterol Nutr 1994 Jul;19(1):27-33
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