<<Disclaimer: Verify this information before applying it to your situation.>>

As a further comment to my previous List posting on food allergies
occurring after going on a GF diet:

http://maelstrom.stjohns.edu/CGI/wa.exe?A2=ind0501e&L=celiac&P=2666

The following study found the lactic acid bacterial strain L. paracasei
NCC2461 induced development of Th3 TGF-beta1 producing T cells when added
to lymphocyte cultures obtained from mice.  Hence this strain may be
particularily useful in a probiotic designed to reduce food allergies.
Though this article does not specifically use the designation "Th3", the
article specifically references the 1994 H.L. Weiner, Science Magazine,
article which first described Th3 cells before given the designation "Th3
cells" in 1996.

Free full text of the article is available at:

http://cdli.asm.org/cgi/content/full/8/4/695?view=full&pmid=11427413

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Clin Diagn Lab Immunol. 2001 Jul;8(4):695-701.

Induction by a lactic acid bacterium of a population of CD4(+) T cells with
low proliferative capacity that produce transforming growth factor beta and
interleukin-10.

von der Weid T, Bulliard C, Schiffrin EJ.

Nestec SA, Nestle Research Center, 1000 Lausanne 26, Switzerland.
[log in to unmask]

We investigated whether certain strains of lactic acid bacteria (LAB) could
antagonize specific T-helper functions in vitro and thus have the potential
to prevent inflammatory intestinal immunopathologies. All strains tested
induced various levels of both interleukin-12 (IL-12) and IL-10 in murine
splenocytes. In particular, Lactobacillus paracasei (strain NCC2461)
induced the highest levels of these cytokines. Since IL-12 and IL-10 have
the potential to induce and suppress Th1 functions, respectively, we
addressed the impact of this bacterium on the outcome of CD4(+) T-cell
differentiation. For this purpose, bacteria were added to mixed lymphocyte
cultures where CD4(+) T-cells from naive BALB/c mice were stimulated weekly
in the presence of irradiated allogeneic splenocytes. In these cultures, L.
paracasei NCC2461 strongly inhibited the proliferative activity of CD4(+) T
cells in a dose-dependent fashion. This was accompanied by a marked
decrease of both Th1 and Th2 effector cytokines, including gamma
interferon, IL-4, and IL-5. In contrast, IL-10 was maintained and
transforming growth factor beta (TGF-beta) was markedly induced in a dose-
dependent manner. The bacteria were not cytotoxic, because cell viability
was not affected after two rounds of stimulation. Thus, unidentified
bacterial components from L. paracasei NCC2461 induced the development of a
population of CD4(+) T cells with low proliferative capacity that produced
TGF-beta and IL-10, reminiscent of previously described subsets of
regulatory cells implicated in oral tolerance and gut homeostasis.

PMID: 11427413 [PubMed - indexed for MEDLINE]

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