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Proteomics. 2003 Jun;3(6):951-6.

http://www3.interscience.wiley.com/cgi-bin/abstract/104539253/START

Identification of new celiac disease autoantigens using proteomic analysis.

Stulik J, Hernychova L, Porkertova S, Pozler O, Tuckova L, Sanchez D, Bures
J.

Proteome Center, Purkyne Military Medical Academy, Hradex Kralove, Czech
Republic.

Celiac disease is an autoimmune disorder in which gluten peptides presented
by specific HLA-DQ2- and HLA-DQ8-positive antigen presenting cells elicit
immune response in connective tissue of lamina propria. Immunoglobulin A
(IgA) antiendomysial antibodies are specific for celiac disease and are
used for screening, diagnosis and follow-up of this disease with an almost
100% sensitivity and specificity. The major target antigen of IgA
antiendomysial antibodies was identified as tissue transglutaminase;
nevertheless, the existence of the additional unique celiac disease-
specific autoantigens is anticipated. In this study we have utilized a
proteomic approach in order to search out new autoantigens recognized by
serum antibodies of patients with active celiac disease. We report the
detection of 11 proteins that were immunorecognized with various
frequencies by sera of patients with celiac disease. Four autoantigens were
identified by mass fingerprinting approach as actin, ATP synthase beta
chain and two charge variants of enolase alpha. While production of IgA
antibodies against actin molecules were described earlier, the existence of
autoantibodies to ATP synthase beta chain and enolase alpha species in sera
collected from patients with active celiac disease are described for the
first time. These results are suggestive of the existence of additional
celiac disease autoantigens with possible diagnostic utility.

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