Page numbers in brackes eg (2) refer to "Enzyme nutrition" by Dr Edward Howell. >>Lynton: >> There might not be enzymatic (?) *activity*, but I would expect >> there to be Lipase (fatty enzymes) present. > >Jean-Louis: >You are assuming that > 1. sunflower seeds contain lipase, Lynton: (147): Fats in the raw state are associated with lipase, whether the fat is animal or vegetable. All seeds contain enzymes, and enzymes multiply during the germination phase >Jean-Loius > 2. enzymes are not removed by the extraction process. > Lynton: (my assumption): Enzymes are way too small to be extracted by normal filtration. (somewhere in the book): Germination will not happen when enzymes have been damaged by heat. (my suggestion): If heat is used then it depends on how hot it gets. If you want to discover a safe heat, try heating a sample of seeds to a known temperature (heat soak), then see how many germinate in comparison with a control sample. Lynton