<<Disclaimer: Verify this information before applying it to your situation.>> Has anyone done a gluten challenge? I was gf for two years with wonderful results. I was so sick back then that my doctors did not do the biopsy because they figure why put me through more discomfort. Well, the symptons are back so in less then two weeks I am having a biospy and a colonscopy. How soon do I have to start eatting gluten????? I am so afraid to get a false negative. Any help would be appreciate because I am so anxious about eatting gluten again and the whole procedure. Thank you! Sabrina Lowrie